An Enzyme-Mediated Aza-Michael Addition Is Involved in the Biosynthesis of an Imidazoyl Hybrid Product of Conidiogenone B
Ranuka T. Hewage, Rou‐Jie Huang, Shu-Jung Lai, Ya-Chu Lien, Shao‐Hsing Weng, Dehai Li, Yu‐Ju Chen, Shih‐Hsiung Wu, Rong‐Jie Chein, Hsiao‐Ching Lin
Abstract
Meleagrin B is a terpene-alkaloid hybrid natural product that contains both the conidiogenone and meleagrin scaffold. Their derivatives show diverse biological activities. We characterized the biosynthesis of (−)-conidiogenone B (1), which involves a diterpene synthase and a P450 monooxygenase. In addition, an α,β-hydrolase (Con-ABH) was shown to catalyze an aza-Michael addition between 1 and imidazole to give 3S-imidazolyl conidiogenone B (6). Compound 6 was more potent than 1 against Staphylococcus aureus strains.
Topics & Concepts
ChemistryDiterpeneBiosynthesisNatural productMichael reactionStereochemistryTerpeneEnzymeImidazoleMonooxygenaseBiochemistryCatalysisCytochrome P450Microbial Natural Products and BiosynthesisPlant biochemistry and biosynthesisPhytochemical compounds biological activities