Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
Jesse J. Waggoner, Victoria Stittleburg, Renee Pond, Youssef Saklawi, Malaya K. Sahoo, Ahmed Babiker, Laila Hussaini, Colleen S. Kraft, Benjamin A. Pinsky, Evan J. Anderson, Nadine Rouphael
Abstract
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
Topics & Concepts
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)CoronavirusMedicine2019-20 coronavirus outbreakBetacoronavirusVirologyReal-time polymerase chain reactionRespiratory systemCoronavirus InfectionsPneumoniaImmunologyInternal medicineBiologyGeneOutbreakGeneticsDiseaseInfectious disease (medical specialty)SARS-CoV-2 detection and testingViral gastroenteritis research and epidemiologySARS-CoV-2 and COVID-19 Research