A novel methyl-dependent DNA endonuclease GlaI coupling with double cascaded strand displacement amplification and CRISPR/Cas12a for ultra-sensitive detection of DNA methylation
Shiying Zhou, Human Sun, Danqun Huo, Xianfeng Wang, Na Qi, Lan Peng, Mei Yang, Peng Lü, Changjun Hou
Topics & Concepts
DNAChemistryEndonucleaseDNA methylationMolecular biologyMultiple displacement amplificationMethylated DNA immunoprecipitationMethylationgenomic DNARestriction enzymeCRISPRPolymerase chain reactionBiologyBiochemistryGeneDNA extractionGene expressionCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniques