Construction of Stable T7 Expression System in Saccharomyces cerevisiae by Improving Nuclear Membrane Permeability with Viroporin HIV-1 Vpu
Kun Yan, Jun Li, Wenya Wang, Qiang Li
Topics & Concepts
EukaryoteT7 RNA polymeraseSaccharomyces cerevisiaeBiologyNuclear poreNuclear transportCytoplasmCell biologyMembrane permeabilityMembrane proteinBiochemistryMolecular biologyBacteriophageMembraneGeneCell nucleusGenomeEscherichia coliBacteriophages and microbial interactionsBacterial Genetics and BiotechnologyProtein purification and stability