N6-methyladenosine levels in peripheral blood RNA: a potential diagnostic biomarker for colorectal cancer
Chunying Zhang, Jiadi Chen, Jingyi Ren, Xiaoyu Li, Yaqin Zhang, Bihan Huang, Yihan Xu, Luyan Dong, Yingping Cao
Abstract
Abstract Background N 6 -methyladenosine (m 6 A) is dysregulated in various cancers, including colorectal cancer (CRC). Herein, we assess the diagnostic potential of peripheral blood (PB) m 6 A levels in CRC. Methods We collected PB from healthy controls (HCs) and patients with CRC, analyzed PB RNA m 6 A levels and the expression of m 6 A-related demethylase genes FTO and ALKBH5 , cocultured CRC cells with PB mononuclear cells (PBMCs), and constructed an MC38 cancer model. Results PB RNA m 6 A levels were higher in the CRC than that in HCs. The area under the curve (AUC) of m 6 A levels (0.886) in the CRC was significantly larger compared with carbohydrate antigen 199 (CA199; 0.666) and carcinoembryonic antigen (CEA; 0.834). The combination of CEA and CA199 with PB RNA m 6 A led to an increase in the AUC (0.935). Compared with HCs, the expression of FTO and ALKBH5 was decreased in the CRC. After coculturing with CRC cells, the PBMCs RNA m 6 A were significantly increased, whereas the expression of FTO and ALKBH5 decreased. Furthermore, m 6 A RNA levels in the PB of MC38 cancer models were upregulated, whereas the expression of FTO and ALKBH5 decreased. Conclusions PB RNA m 6 A levels are a potential diagnostic biomarker for patients with CRC.