Glucose level determination in single cells in their satiety and starvation states using an enzymatic functional glass nanopore
Dandan Wang, Guohua Qi, Ya Zhou, Haijuan Li, Ying Zhang, Xu Chen, Ping Hu, Yongdong Jin
Abstract
A cytochrome c (Cyt c)-modified glass nanopore sensing platform was constructed to sensitively detect glucose in single cells based on changes in the ionic current rectification (ICR) of the system. A difference in glucose content between single H8 and HeLa cells in their satiety or starvation states was clearly able to be detected using this method.
Topics & Concepts
NanoporeEnzymeChemistrySubstrate (aquarium)Ionic bondingBiophysicsBiochemistryMaterials scienceNanotechnologyBiologyIonOrganic chemistryEcologyNanopore and Nanochannel Transport StudiesElectrochemical Analysis and ApplicationsElectrochemical sensors and biosensors