Ribosomal incorporation of cyclic β-amino acids into peptides using <i>in vitro</i> translation
Joongoo Lee, Rafael Torres, Do Soon Kim, Michelle Byrom, Andrew D. Ellington, Michael C. Jewett
Abstract
We demonstrate in vitro incorporation of cyclic β-amino acids into peptides by the ribosome through genetic code reprogramming. Further, we show that incorporation efficiency can be increased through the addition of elongation factor P.
Topics & Concepts
Translation (biology)In vitroAmino acidChemistryRibosomal RNARibosomeBiochemistryCombinatorial chemistryRNAMessenger RNAGeneRNA and protein synthesis mechanismsChemical Synthesis and AnalysisRNA modifications and cancer