Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene-derived TiO<sub>2</sub>/C-QDs as oxidase mimics for the efficient diagnosis of glutathione in human serum
Zhaoyong Jin, Gengfang Xu, Yusheng Niu, Xiaoteng Ding, Yaqian Han, Wenhan Kong, Yanfeng Fang, Haitao Niu, Yuanhong Xu
Abstract
Nanozyme-based colorimetry was suggested to be a rapid method for biomarker (e.g. glutathione) detection, but this method suffers from lack of efficiency and low-toxicity nanozymes till now. Herein, quantum dots of TiO2 loaded on carbon (TiO2/C-QDs) oxidase-like nanozymes were prepared via a hydrothermal treatment of tiny and few-layered Ti3C2Tx MXene nanosheets, which possess abundant thermodynamic metastable Ti atoms on MXene margins as raw materials for the preparation of TiO2/C-QDs. The oxygen vacancy in TiO2 on the surface of the carbon matrix can facilitate O2 adsorption in the solution and generate reactive oxygen species (ROS), thereby quickly oxidizing 3,3',5,5'-tetramethylbenzidine (TMB) to its oxidized form (TMBox) in the absence of H2O2. After adding glutathione (GSH), TMBox was able to be restored to TMB, which resulted in a corresponding decrease in the UV-vis absorbance value at 652 nm. Furthermore, this assay possesses good selectivity, excellent specificity and high sensitivity (limit of detection: 0.2 μM), which made it possible to efficiently detect GSH in complex biological samples such as human serum.