Liposome manufacturing under continuous flow conditions: towards a fully integrated set-up with in-line control of critical quality attributes
Maryam Sheybanifard, Luis P. B. Guerzoni, Abdolrahman Omidinia‐Anarkoli, Laura De Laporte, Johannes F. Buyel, R. Besseling, Michiel Damen, Ad Gerich, Twan Lammers, Josbert M. Metselaar
Abstract
, above 20% v/v) emerged as critical parameters to tailor liposome size between 80 and 150 nm. After liposome formation, the pressure and the surface area of the filter in the ultrafiltration unit were critical parameters in the process of clearing the dispersion from residual ethanol. As a final step, we integrated in-line measurement of liposome size and residual ethanol content. Such in-line measurements allow for real-time monitoring and in-process adjustment of key composition and process parameters.
Topics & Concepts
Quality (philosophy)Continuous flowControl (management)LiposomeFlow (mathematics)Set (abstract data type)Line (geometry)Process engineeringComputer scienceManufacturing engineeringEngineeringBiochemical engineeringMaterials scienceMathematicsArtificial intelligenceNanotechnologyEpistemologyPhilosophyProgramming languageGeometryNanoparticle-Based Drug DeliveryInnovative Microfluidic and Catalytic Techniques InnovationAdvanced biosensing and bioanalysis techniques