Methanofollis propanolicus sp. nov., a novel archaeal isolate from a Costa Rican oil well that uses propanol for methane production
Linda Dengler, Julia Meier, Felix Grünberger, Annett Bellack, Reinhard Rachel, Dina Grohmann, Harald Huber
Abstract
Abstract A novel methanogenic strain, CaP3V-MF-L2A T , was isolated from an exploratory oil well from Cahuita National Park, Costa Rica. The cells were irregular cocci, 0.8–1.8 μm in diameter, stained Gram-negative and were motile. The strain utilized H 2 /CO 2 , formate and the primary and secondary alcohols 1-propanol and 2-propanol for methanogenesis, but not acetate, methanol, ethanol, 1-butanol or 2-butanol. Acetate was required as carbon source. The novel isolate grew at 25–40 °C, pH 6.0–7.5 and 0–2.5% (w/v) NaCl. 16S rRNA gene sequence analysis revealed that the strain is affiliated to the genus Methanofollis . It shows 98.8% sequence similarity to its closest relative Methanofollis ethanolicus . The G + C content is 60.1 mol%. Based on the data presented here type strain CaP3V-MF-L2A T (= DSM 113321 T = JCM 39176 T ) represents a novel species, Methanofollis propanolicus sp. nov.