Modified multilayered porous titanium scaffolds with silicon-doped coating surface-loaded BMP-2 prepared by microarc oxidation for bone defect repair
X. L. Zhang, Xu Zhang, Wuchao Zhou, MengZhen Tang, Tao Wang, Khanh Le, Weihong Xi
Abstract
As is generally known, the lack of osteoinductive properties in titanium scaffolds has been a major barrier to bone defects repair. In our previous studies, we found that silicon-doped porous coatings prepared via micro-arc oxidation (MAO) had the potential application of Si-doped coatings in dental and orthopedic fields. We are currently focused on developing more advanced surface coatings to maximize their osteoinductive efficacy. Bone morphogenetic protein-2 (BMP-2), known for its ability to induce bone and cartilage development, has also been extensively studied. Therefore, in this study, macroporous titanium alloy scaffolds were fabricated using 3D printing technology, silicon ions were introduced onto the surface of customized macroporous titanium alloy scaffolds through MAO to enhance the bioactivity of the scaffolds, and BMP-2 was loaded onto the Si-doped coating to further improve the osseointegration of the titanium alloy scaffolds post-implantation. Results demonstrated that silicon-doped coating-loaded BMP2-modified multi-level porous titanium scaffolds exhibited improved hydrophilicity and biocompatibility. In-vivo and vitro studies further confirmed the superior bone regeneration capabilities of these scaffolds. This study demonstrates an optional strategy to combine two surface modification treatments, offering substantial potential as an advanced bone defect repair material with improved and accelerated bone regeneration. • The multistage pore-like structure of macro/micropores coexistence can better promote the adhesion of value-added cells. • The Si-doped coating prepared by micro-arc oxidation can stably release Si ions and promote cellular osteogenesis and vascular differentiation. • The introduction of Si ions and BMP2 in combination with two surface modification techniques improved the bioactivity of titanium alloys.