Multilocus sequence analysis of endophytic fungi from Justicia brandegeana with the culture-dependent method and their bioprospection for health field
Angela Aparecida da Silva, Júlio César Polonio, João Arthur dos Santos Oliveira, Aline Maria Bulla, Halison Correia Golias, Andressa Domingos Polli, Lilian Capelari Soares, Verônica Elisa Pimenta Vicentini, Arildo José Braz de Oliveira, José Eduardo Gonçalves, Regina Aparecida Correia Gonçalves, João Lúcio de Azevedo, Benício Alves de Abreu Filho, João Alencar Pamphile
Abstract
The endophytic fungi live inside plant tissues and do not develop any disease to their hosts. These microorganisms can produce bioactive compounds. In this work, we aimed to evaluate the leaf endophytic fungi microbiota from J. brandegeana using the culture-dependent method and multilocus sequence analysis. The endophyte's isolation was performed by the fragmentation method and the MLSA phylogeny was made using ITS, elongation factor 1-α, β-tubulin, glyceraldehyde-3-phosphate and, actin genes. For the screening selection, agar piece test was performed and, amongst the strains, JB06 isolate was chosen for metabolic extraction from the fermented culture and the evaluation of the antibacterial, antioxidant, antiproliferative activities and, phenolic compounds assay. Through the MLSA analysis, there were five genera of fungi identified, with Colletotrichum the higher isolated. For the antioxidant assay using the fungal extract obtained from Mycoleptodiscus indicus (JB06), the best results were obtained with the ABTS method. Antiproliferative effects by the crude extract of the endophyte against the tumor hepatocellular carcinoma cells (HepG2/C3A) were found. In summary, our findings revealed highly diverse endophytic fungi associated with J. brandegeana, with the bioprospection of the endophyte M. indicus with biotechnological potential for the health field.