Direct Identification of Proteolytic Cleavages on Living Cells Using a Glycan-Tethered Peptide Ligase
Kaitlin Schaefer, Irene Lui, James R. Byrnes, Emily L. Kang, Jie Zhou, Amy M. Weeks, James A. Wells
Abstract
, induce extracellular proteolytic remodeling of proteins involved in cancerous cell growth, invasion, and migration. Cell surface N-terminomics is a generalizable platform that can reveal proteolyzed, neoepitopes to target using immunotherapies.
Topics & Concepts
GlycanProteasesExtracellularCell biologyCleavage (geology)CellBiologyBiotinylationExtracellular matrixCell migrationChemistryBiochemistryGlycoproteinEnzymeFracture (geology)PaleontologyPeptidase Inhibition and AnalysisUbiquitin and proteasome pathwaysCell Adhesion Molecules Research