Multispectral compressive fluorescence lifetime imaging microscopy with a SPAD array detector
Alberto Ghezzi, Andrea Farina, Andrea Bassi, Gianluca Valentini, Ivan Labanca, Giulia Acconcia, Ivan Rech, Cosimo D’Andrea
Abstract
Multispectral/hyperspectral fluorescence lifetime imaging microscopy ( <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"> <mml:mi>λ</mml:mi> </mml:math> FLIM) is a promising tool for studying functional and structural biological processes. The rich information content provided by a multidimensional dataset is often in contrast with the acquisition speed. In this work, we develop and experimentally demonstrate a wide-field <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"> <mml:mi>λ</mml:mi> </mml:math> FLIM setup, based on a novel time-resolved <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"> <mml:mn>18</mml:mn> <mml:mo>×</mml:mo> <mml:mn>1</mml:mn> </mml:math> single-photon avalanche diode array detector working in a single-pixel camera scheme, which parallelizes the spectral detection, reducing measurement time. The proposed system, which implements a single-pixel camera with a compressive sensing scheme, represents an optimal microscopy framework towards the design of <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"> <mml:mi>λ</mml:mi> </mml:math> FLIM setups.