Single-shot wavelength-multiplexed digital holography for 3D fluorescent microscopy and other imaging modalities
Tatsuki Tahara, A. Ishii, Tomoyoshi Ito, Yasuyuki Ichihashi, Ryutaro Oi
Abstract
We propose a holographic technique by which multiwavelength three-dimensional spatial information is obtained with both a single-shot exposure of a monochrome image sensor and an in-line holographic multiplexing scheme. We develop a specially designed monochrome image sensor with a wavelength-dependent phase-modulation array, which is used to obtain the holographic information required for the proposed technique. The proposed technique is applicable for not only laser holography but also spatially and temporally incoherent holography. Its validity is experimentally demonstrated by constructing a color-multiplexed fluorescence digital holographic microscope and a multicolor incoherent digital holography system with a white-light source.