Development of Spectral Imaging Cytometry
Ivan A. Vorobjev, Aigul Kussanova, Natasha S. Barteneva
Abstract
Spectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells-from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry.
Topics & Concepts
AutofluorescenceFlow cytometryFluorescenceSpectral imagingHyperspectral imagingCytometrySpectral analysisSpectral propertiesBiological systemMaterials scienceOpticsChemistryRemote sensingBiologyPhysicsSpectroscopyMolecular biologyGeologyComputational chemistryQuantum mechanicsSingle-cell and spatial transcriptomicsMicrofluidic and Bio-sensing Technologies