Agonist-induced phosphorylation of orthologues of the orphan receptor GPR35 functions as an activation sensor
Nina Divorty, Laura Jenkins, Amlan Ganguly, Adrian J. Butcher, Brian D. Hudson, Stefan Schulz, Andrew B. Tobin, Stuart A. Nicklin, Graeme Milligan
Abstract
in mouse) GPR35a identified only the mature forms of GPR35 and provided effective sensors of the activation status of the receptors both in immunoblotting and immunocytochemical studies. Such antisera may be useful tools to evaluate target engagement in drug discovery and target validation programs.
Topics & Concepts
AgonistOrphan receptorReceptorBiologySerinePhosphorylationG protein-coupled receptorCell biologyMutantMolecular biologyGeneBiochemistryTranscription factorReceptor Mechanisms and SignalingMass Spectrometry Techniques and ApplicationsNeuropeptides and Animal Physiology