Topography induced stiffness alteration of stem cells influences osteogenic differentiation
Liangliang Yang, Qi Gao, Lu Ge, Qihui Zhou, Eliza M. Warszawik, Reinier Bron, King Wai Chiu Lai, Patrick van Rijn
Abstract
elicited from different topographies, resulted in highly altered differentiation behavior and the underlying single cell mechanics was found to be critical. The cell behavior was found to be focal adhesion-mediated and induced stiffness alterations rather than just influencing the cell elongation. The effect was further corroborated by investigations of the transcriptional regulators YAP. Our study provides insight into how mechanical properties of the cell, which are stimulated by topography, modulate the osteogenesis of hBM-MSCs, which is beneficial for improving the understanding of interactions between stem cells and topography for developing applications of tissue engineering and regenerative medicine.