Structure, nitric oxide (NO) generation and antitumor activity of binuclear tetranitrosyl iron complex with 4-aminothiophenolyl as nitrosyl ferredoxins mimic
Н. А. Санина, Г. И. Козуб, T.A. Kondrat’eva, Tatyana S. Stupina, А. А. Балакина, А. А. Терентьев, И. В. Сулименков, N. S. Ovanesyan, Павел В. Дороватовский, Victor N. Khrustalev, С. М. Алдошин
Abstract
Single-crystal XRD structure of binuclear tetranitrosyliron complex [Fe2(SR)2(NO)4] (1), R = 4-aminothiophenyl, has been described. The properties of 1 were studied by IR, Mössbauer, mass spectroscopy, and amperometry. Complex 1 was shown to form long-living nitrosyl intermediates in solution, which underlies its prolonged NO donor activity. In HeLa cells, 1 caused accumulation of NF-κB p50 but decreased the levels of NF-κB p65 in the nucleus, which led to inhibition of NF-κB dependent genes. The complex also induced increase of p53 protein levels and p21 gene activity, which led to the apoptotic cell death after G2/M cell cycle phase.