Essential Role for an Isoform of Escherichia coli Translation Initiation Factor IF2 in Repair of Two-Ended DNA Double-Strand Breaks
Jillella Mallikarjun, J. Gowrishankar
Abstract
Double-strand breaks (DSBs) in DNA are major threats to genome integrity. In Escherichia coli, DSBs are repaired by RecA- and RecBCD-mediated homologous recombination (HR). This study demonstrates a critical role for an isoform (IF2-1) of the translation initiation factor IF2 in the repair of two-ended DSBs in E. coli (that can be generated by ionizing radiation, certain DNA-damaging chemicals, or endonuclease action). It is proposed that IF2-1 acts to facilitate the function of RecA in the synapsis between a pair of DNA molecules during HR.
Topics & Concepts
BiologyRecBCDHomologous recombinationEscherichia coliDNA repairDNAGeneticsInitiation factorDNA mismatch repairMolecular biologyCell biologyTranslation (biology)GeneMessenger RNADNA Repair MechanismsBacterial Genetics and BiotechnologyCRISPR and Genetic Engineering