DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs
Chen Liu, Jialun Han, Lujian Zhou, Jingjing Zhang, Jie Du
Abstract
MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L−1 level hence affording a simple, highly sensitive, and selective low cost detection platform.
Topics & Concepts
DeoxyribozymeRolling circle replicationmicroRNASuppressorDNAChemistryFluorescenceCell biologyBiologyGeneComputational biologyMolecular biologyBiochemistryPhysicsDNA polymeraseQuantum mechanicsAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryBiosensors and Analytical Detection