Highly sensitive reporter cell line for detection of interferon types I–III and their neutralization by antibodies
Kevin Groen, Roger Kuratli, Lauren Sar, Andri Vasou, Michael Huber, David J. Hughes, Benjamin G. Hale
Abstract
Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity to IFNs than a commonly used alternative biological reporter system and allows for a rapid and simple live-cell workflow for detecting low titer amounts of neutralizing anti-IFN antibodies.
Topics & Concepts
BiologyNeutralizationAntibodyVirologyInterferonCell cultureMolecular biologyImmunologyGeneticsinterferon and immune responsesImmune Response and InflammationImmunotherapy and Immune Responses