High-throughput differential scanning fluorimetry (DSF) and cellular thermal shift assays (CETSA): Shifting from manual to automated screening
Catherine S. Hansel, Alice Lanne, Hannah Rowlands, Joseph Shaw, Matthew Collier, Helen Plant
Abstract
Biophysical affinity screening is increasingly being adopted as a high-throughput hit finding technique in drug discovery. Automation is highly beneficial to high-throughput screening (HTS) since a large number of compounds need to be reproducibly tested against a biological target. Herein, we describe how we have automated two biophysical affinity screening methods that rely on a thermal shift in protein melting temperature upon small molecule binding: differential scanning fluorimetry (DSF) and the cellular thermal shift assay (CETSA).
Topics & Concepts
High-throughput screeningThroughputDrug discoveryFluorescence spectroscopyFluorescenceChemistryBiophysicsComputational biologyNanotechnologyMaterials scienceBiologyBiochemistryComputer sciencePhysicsWirelessTelecommunicationsQuantum mechanicsProtein Structure and DynamicsComputational Drug Discovery MethodsMonoclonal and Polyclonal Antibodies Research