Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase
Hidehiko Kumagai, Takane Katayama, Takashi Koyanagi, Hideyuki Suzuki
Abstract
L-DOPA is an amino acid that is used as a treatment for Parkinson's disease. A simple enzymatic synthesis method of L-DOPA had been developed using bacterial L-tyrosine phenol-lyase (Tpl). This review describes research on screening of bacterial strains, culture conditions, properties of the enzyme, reaction mechanism of the enzyme, and the reaction conditions for the production of L-DOPA. Furthermore, molecular bleeding of constitutively Tpl-overproducing strains is described, which were developed based on mutations in a DNA binding protein, TyrR, which controls the induction of tpl gene expression.
Topics & Concepts
EnzymeBiochemistryTyrosinePhenolGeneChemistryBiosynthesisAmino acidBiologyOrganic chemistryMetabolism and Genetic DisordersEnzyme Structure and FunctionMicrobial Metabolic Engineering and Bioproduction