Multimodal chemical imaging of a single brain tissue section using ToF-SIMS, MALDI-ToF and immuno/histochemical staining
Ibrahim Kaya, Eva Jennische, Stefan Lange, Per Malmberg
Abstract
Cluster ion beam ToF-SIMS and/or MALDI-ToF mass spectrometry imaging (using 1,5-DAN matrix via sublimation) of a single coronal rat brain tissue section followed by classical- or immuno- histochemical staining faclilated a new multimodal chemical imaging workflow allowing complementary correlation of the lipid molecular ion images with the immuno/histological features within cerebellum region of the same brain tisue section.
Topics & Concepts
StainingChemistryMALDI imagingMass spectrometry imagingBrain tissueMass spectrometryPathologyMatrix-assisted laser desorption/ionizationChromatographyBiomedical engineeringMedicineOrganic chemistryDesorptionAdsorptionIon-surface interactions and analysisMass Spectrometry Techniques and ApplicationsForensic Fingerprint Detection Methods