A New Approach for Nitrogen Status Monitoring in Potato Plants by Combining RGB Images and SPAD Measurements
Huanbo Yang, Yaohua Hu, Zhouzhou Zheng, Yichen Qiao, Bingru Hou, Jun Chen
Abstract
Precise nitrogen (N) application ensures the best N status of potato plants to improve crop growth and food quality and to achieve the best N use efficiency. Four N fertilization levels (0, 2, 4 and 6 g N pot−1) were used to establish a critical N dilution curve (CNDC) of potato plants cultivated in substrates with a greenhouse environment. RGB images of potato plants were obtained, and a red–green fit index (RGFI) was calculated based on the linear relationship between R and G channels and the principle of the excess green index (EXG). The N in the substrate can meet the nutritional requirements of potato plants during the first 35 days after emergence. In order to solve the complex sampling problem of maintaining a sufficient N strip for aboveground dry biomass (DM) and crop nitrogen concentration, a reference curve method for detecting N status was proposed. RGFI and SPAD values from the economically optimum 4 g N pot−1 treatment were used to derive the reference curve. The RGFI and SPAD values from the 4 g N pot−1 treatment had high correlations and were fitted with a second-order polynomial function with an R2 value of 0.860 and an RMSE value of 2.10. The validation results show that the N concentration dilution curve constructed by RGFI and SPAD values can effectively distinguish N-limiting from non-N-limiting treatments, CNDCs constructed based on RGFI and SPAD values could be used as an effective N status monitoring tool for greenhouse potato production.