Porcine Deltacoronavirus Enters Porcine IPI-2I Intestinal Epithelial Cells via Macropinocytosis and Clathrin-Mediated Endocytosis Dependent on pH and Dynamin
Puxian Fang, Jiansong Zhang, Huichang Zhang, Sijin Xia, Jie Ren, Liyuan Tian, Dongcheng Bai, Liurong Fang, Shaobo Xiao
Abstract
. Here, we demonstrate that PDCoV enters IPI-2I cells via macropinocytosis, which does not require a specific receptor, and clathrin-mediated endocytosis, which requires a low-pH environment and dynamin, while a caveola-mediated endocytic pathway is used by PDCoV to enter swine testicular (ST) cells and porcine kidney (LLC-PK1) cells. These findings provide a molecular detail of the cellular entry pathways of PDCoV and may direct us toward novel antiviral drug development.
Topics & Concepts
PinocytosisBiologyEndocytosisEndocytic cycleCell biologyIntestinal epitheliumViral entryCaveolaeCoronavirusClathrinCell cultureSignal transductionIntestinal mucosaPathogenesisEpitheliumVirologyTight junctionGastrointestinal epitheliumDynaminVacuoleIntracellularMicrovesiclesEndosomeAnimal Virus Infections StudiesSARS-CoV-2 and COVID-19 ResearchXenotransplantation and immune response