A strategy to assess the cellular activity of E3 ligase components against neo-substrates using electrophilic probes
Benika J. Pinch, Dennis L. Buckley, Scott Gleim, Scott M. Brittain, Laura Tandeske, Pier Luca D’Alessandro, Zachary J. Hauseman, Jennifer Lipps, Lei Xu, Edward P. Harvey, Markus Schirle, Elizabeth R. Sprague, William C. Forrester, Dustin Dovala, Lynn M. McGregor, Claudio R. Thoma
Topics & Concepts
Ubiquitin ligaseDNA ligaseCullinUbiquitinBiochemistryCell biologyDDB1ChemistryBiologyEnzymeGeneProtein Degradation and InhibitorsUbiquitin and proteasome pathwaysPeptidase Inhibition and Analysis