Improving the knock-in efficiency of the MOF-encapsulated CRISPR/Cas9 system through controllable embedding structures
Chang Liu, Xiaoyu Xu, Oliver Koivisto, Wenhui Zhou, Guillaume Jacquemet, Jessica M. Rosenholm, Hongbo Zhang
Abstract
assays it was found that the superior MOF vector can greatly enhance cellular endocytosis and endo/lysosomal escape of sheltered plasmids, resulting in successful knock-in of GFP-tagged paxillin genomic sequences in cancer cell lines with high transfection potency compared to our previous studies. Thus, the development of new cost-effective approaches for MOF-based intracellular delivery systems offers an attractive option for overcoming the physiological barriers to CRISPR/Cas9 delivery, which shows great potential for investigating paxillin-associated focal adhesions and signal regulation.
Topics & Concepts
CRISPRPlasmidCas9TransfectionNanotechnologyPaxillinChemistryMaterials scienceBiophysicsComputational biologyCell biologyBiologyGeneSignal transductionFocal adhesionBiochemistryAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryCRISPR and Genetic Engineering