Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytes
Geoffrey L. Rogers, Chun Huang, Robert D.E. Clark, Eduardo Seclén, Hsu-Yu Chen, Paula M. Cannon
Abstract
genome editing with AAV6 vectors and provide a blueprint for improving AAV6-mediated homology-directed editing of human T and B cells.
Topics & Concepts
Transduction (biophysics)Genome editingBiologyEx vivoElectroporationHaematopoiesisCell biologyGenomeComputational biologyMolecular biologyGeneticsIn vivoStem cellGeneBiochemistryCRISPR and Genetic EngineeringVirus-based gene therapy researchCytomegalovirus and herpesvirus research