Engineered cell-overexpression of circular RNA hybrid hydrogels promotes healing of calvarial defects
Min Zhang, Yanjing He, Xu Zhang, Shuaiqi Gan, Xi Xie, Zheng Zheng, Jinfeng Liao, Wenchuan Chen
Abstract
showed that the overexpressing circ-CTTN (OE group) exhibits a more pronounced osteogenic phenotype. The levels of osteogenesis-related genes in the OE group were highly expressed. The gelatin-methacrylate (GelMA) hydrogel possessed excellent biocompatibility and was used to load hUCMSCs. In the rat calvarial defect, the OE group presented a larger bone healing volume and denser bone trabecular distribution than other groups. So far, the overexpression of circ-CTTN could enhance the osteogenic differentiation of hUCMSCs and accelerate bone reconstruction. Our research could provide a new strategy and a strong theoretical basis for promoting hUCMSC clinical application in bone tissue engineering.