Safe-Harboring based novel genetic toolkit for Nannochloropsis salina CCMP1776: Efficient overexpression of transgene via CRISPR/Cas9-Mediated Knock-in at the transcriptional hotspot
Ae Jin Ryu, Byeong‐ryool Jeong, Nam Kyu Kang, Seungjib Jeon, Min Gi Sohn, Hyo Jin Yun, Jong Min Lim, Seok Won Jeong, Youn‐Il Park, Won Joong Jeong, Sunghoon Park, Yong Keun Chang, Ki Jun Jeong
Topics & Concepts
TransgeneExpression cassetteBiologyCas9MutantCRISPRPhytoene desaturaseGenetic screenGreen fluorescent proteinGeneFatty acid desaturaseCell biologyGene silencingFatty acidGeneticsBiochemistryRecombinant DNAVector (molecular biology)Polyunsaturated fatty acidMicrobial Metabolic Engineering and BioproductionAlgal biology and biofuel productionViral Infectious Diseases and Gene Expression in Insects