Differentiation of intraspecific phenotypic plasticity of elongate glassy perchlet, Chanda nama: Insights into landmark-based truss morphometric and meristic variations
Kamrun Naher Azad, Sarower Mahfuj, Towsif Iqbal, Khairun Azad, Md Shafaq
Abstract
OBJECTIVE: - Jashore (JBJ) in Bangladesh. MATERIALS AND METHODS: In this study, six meristics, 15 conventional morphometrics, and 23-truss-based morphometrics were subjected to a one-way analysis of variance (ANOVA), followed by the Tukey-HSD test. The mean values of three meristic counts and nine conventional and 12 truss-based morphometrics demonstrated significant differences in the ANOVA test. Furthermore, principal component analysis (PCA) and discrimination function analysis (DFA) were performed separately using conventional and truss-based morphometric data. RESULTS: In PCA analysis, four principal components were extracted and cumulatively demonstrated 51.848%. On the contrary, two discriminant functions (DF1, 63.5%; DF2, 36.5%) resulted from DFA analysis. In the bi-plot alignment from the discriminant space, all individuals were exceedingly separated among the three inhabitants. A dendrogram developed using conventional and truss morphometric characters confirmed that two clusters were formed among three populations. The TRJ population formed a distinct cluster, and the JBJ population formed a different cluster with a subcluster of MRN. In the discriminant function analysis, precise classification outcomes displayed 82% of individuals into their unique populations, whereas 66.9% of individuals were categorized as a cross-validated assemblage. CONCLUSION: The baseline information resulting from the current study would be useful for environmental studies and further conservation of glassy perchlet populations in Bangladesh.