CRISPR/Cas12a-mediated ultrasensitive and on-site monkeypox viral testing
Furong Zhao, Pei Wang, Haoxuan Wang, Sirui Liu, Muhammad Sohail, Xing Zhang, Bingzhi Li, He Huang
Abstract
copies per μL. Our RAA-Cas12a-MPXV assay was able to detect MPXV without the need for sophisticated operation and expensive equipment. We believe that this assay can be rapidly deployed in emerging viral outbreaks for on-site surveillance of MPXV.
Topics & Concepts
MonkeypoxCRISPRVirologyComputational biologyBiologyGeneticsVacciniaGeneRecombinant DNAPoxvirus research and outbreaksPlant Virus Research StudiesHerpesvirus Infections and Treatments