Quantification of Severe Acute Respiratory Syndrome Coronavirus 2 Binding Antibody Levels To Assess Infection and Vaccine-Induced Immunity Using WHO Standards
Olivier Pernet, Steven Balog, Eric S. Kawaguchi, Chun Nok Lam, Patricia Anthony, Paul Simon, Rani Kotha, Neeraj Sood, Howard Hu, Andrea Kovács
Abstract
Accurate quantification of SARS-CoV-2-specific antibodies can be achieved using a universal standard with sample dilution within the linear range. With hybrid immunity being now common, it is critical to use protocols adapted to high Ab levels to standardize serological results. We validated this approach with the Los Angeles Pandemic Surveillance Cohort by comparing the antibody decay rates in vaccinated participants and vaccinated infected participants.
Topics & Concepts
SerologyVaccinationAntibodyMedicineImmunologyImmunityCohortRespiratory systemVirologyInternal medicineImmune systemSARS-CoV-2 and COVID-19 ResearchCOVID-19 Clinical Research StudiesSARS-CoV-2 detection and testing