Kaposi’s Sarcoma-Associated Herpesvirus-Encoded circRNAs Are Expressed in Infected Tumor Tissues and Are Incorporated into Virions
Bizunesh Abere, Jinghui Li, Hongzhao Zhou, Tuna Toptan, Patrick S. Moore, Yuan Chang
Abstract
KSHV has recently been found to encode circRNAs. circRNAs result from back-splicing of an upstream pre-mRNA splice donor exon-intron junction to an acceptor site, generating a covalently closed circle. This study revealed that for one KSHV region, the PAN/K7.3 locus, broadly and bidirectionally generated circRNA levels parallel corresponding linear RNA levels. Another KSHV circularization site (circ-vIRF4), however, showed expression that differed from that of the corresponding linear RNA. All KSHV circRNAs are incorporated into KSHV virions and are potentially expressed as immediate early products in newly infected cells.
Topics & Concepts
BiologyVirusVirologyCytoplasmRNAPrimary effusion lymphomaMolecular biologyCell culturePolyadenylationCellOncovirusEpstein–Barr virusGene expressionInterferonLocus (genetics)Circular RNAGeneMessenger RNAViral replicationRNA virusCell biologyVector (molecular biology)LymphomaTumor VirusGammaherpesvirinaeSubcellular localizationViral-associated cancers and disordersHerpesvirus Infections and TreatmentsCytomegalovirus and herpesvirus research