<i>In vivo</i> mRNA imaging based on tripartite DNA probe mediated catalyzed hairpin assembly
Han Wu, Wenjing Zhou, Lan Liu, Ze Fan, Hao Tang, Ru‐Qin Yu, Jian‐Hui Jiang
Abstract
Herein, we develop a novel tripartite DNA probe to transport phosphorothioated substrate hairpins and an aptamer for the intramolecular CHA circuit, which achieves detection of a low amount of specific mRNA in living cells and mice. Our study provides an improved strategy to promote in vivo fluorescence imaging applications in early-stage clinical diagnosis.
Topics & Concepts
DNAIn vivoMessenger RNAIntramolecular forceAptamerChemistryBiophysicsSubstrate (aquarium)CatalysisCell biologyMolecular biologyComputational biologyBiologyBiochemistryGeneStereochemistryGeneticsEcologyAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryRNA Interference and Gene Delivery