A CRISPR/Cas9-Mediated, Homology-Independent Tool Developed for Targeted Genome Integration in Yarrowia lipolytica
Zhiyong Cui, Huihui Zheng, Jinhong Zhang, Zhennan Jiang, Ziwei Zhu, Xiaoqin Liu, Qingsheng Qi, Jin Hou
Abstract
This study describes the development and optimization of a homology-independent targeted genome integration tool mediated by CRISPR/Cas9 in Yarrowia lipolytica . This tool does not require the construction of homologous templates and can be used to rapidly verify genetic elements and to iteratively integrate multiple-gene pathways in Y. lipolytica . This tool may serve as a potential supplement to current HR-dependent genome-editing methods for eukaryotes.
Topics & Concepts
YarrowiaCRISPRGenome editingGenomeCas9Computational biologyBiologyGeneHomology (biology)Genome engineeringGeneticsHomologous recombinationCRISPR and Genetic EngineeringMicrobial Metabolic Engineering and BioproductionRNA and protein synthesis mechanisms