Exploration of Antioxidant, Antibacterial Activities of Green Synthesized Hesperidin Loaded PLGA Nanoparticles
Krithiga Balakrishnan, Casimeer C. Sangeetha, Alaa Yousef Ghidan, Tawfiq Al Antary, Anbu Singaravelu, D Rusic, J Bukic, A Seselja Perisin, D Leskur, D Modun, A Petric, M Vilovic, J Bozic, J Martinez, T Coque, F Baquero, M Cheesman, A Ilanko, B Blonk, I Cock, A Mariadoss, R Vinyagam, V Rajamanickam, V Sankaran, S Venkatesan, E David, A Mariadoss, R Vinayagam, B Xu, K Venkatachalam, V Sankaran, S Vijayakumar, S Bakthavatsalam, S Mohamed, E David, K Venkatachalam, R Vinayagam, M Arokia Vijaya Anand, N Isa, R Ponnaiyan, E Atta, N Mohamed, A Abdelgawad, S Ali, H Ahsan, M Zia, T Siddiqui, F Khan, S Rafiq, R Kaul, S Sofi, N Bashir, F Nazir, G Nayik, L Yi, S Ma, D Ren, A Mahmoud, R Hernandez Bautista, M Sandhu, O Hussein, S Srinivasan, V Vinothkumar, R Murali, D Stanisic, L Liu, R Santos, A Costa, N Durn, L Tasic, Y Park, K Do, S Bok, M Lee, T Jeong, M Choi, V Aggarwal, H Tuli, F Thakral, P Singhal, D Aggarwal, S Srivastava, A Pandey, K Sak, M Varol, M Khan, G Sethi, R Rezaee, A Sheidary, S Jangjoo, S Ekhtiary, S Bagheri, Z Kohkan, M Dadres, A Oana Docea, K Tsarouhas, D Sarigiannis, S Karakitsios, R Zaghloul, N Elsherbiny
Abstract
To elaborate on the potential benefits of hesperidin loaded PLGA nanoparticles (HES-PLGA NPs), present investigation reports about the antioxidant, antimicrobial, and cytotoxic potential of hesperidin loaded PLGA nanoparticles (HES-PLGA-NPs). The antioxidant potential of Hesperidin and HES-PLGA-NPs was demonstrated by ATBS, DPPH, Hydroxy, Hydrogen peroxide, and superoxide radical showing the dose-dependent antioxidants activity. Both the test gained better potential with HES loaded PLGA nanoparticles than free HES. HES-PLGA-NPs also proved its antibacterial activity against tested bacterial strains, including Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogenes, and Pseudomonas aeruginosa. HES-PLGA-NPs had a good ability to minimize biofilms' formation and a good to weak ability to degrade pre-formed biofilms. Thus, the bionanotherapy, which has been attempted using HES-PLGA-NPs would provide a promising scope as a potent bio compound that can be replaced for medical ailments in a cost-effective, greener synthesis.