Solvent accessibility changes in a Na+-dependent C4-dicarboxylate transporter suggest differential substrate effects in a multistep mechanism
Connor D. D. Sampson, Matthew J. Stewart, Joseph A. Mindell, Christopher Mulligan
Abstract
binding drives the transporter into an as-yet-unidentified conformational state, involving rearrangement of the substrate-binding site-associated re-entrant hairpin loops. These findings provide insight into the mechanism of VcINDY, which is currently the only structurally characterized representative of the entire DASS family.
Topics & Concepts
Mechanism (biology)Substrate (aquarium)ChemistryDifferential (mechanical device)TransporterSolventBiophysicsStereochemistryBiochemistryBiologyPhysicsGeneThermodynamicsQuantum mechanicsEcologyDrug Transport and Resistance MechanismsDNA and Nucleic Acid ChemistryAmino Acid Enzymes and Metabolism