“Turn-on” NIR-II fluorescence of a dually quenched probe for sensitive imaging of cathepsin B <i>in vivo</i>
Yu Deng, Kui Yan, Lingling Xu, Xiaoyang Liu, Furong Zhao, Shangfeng Wang, Fan Zhang, Gaolin Liang, Rui Wang
Abstract
Nanoparticle AF3-NP with dually quenched NIR-II fluorescence was rationally designed. Upon uptake by cancer cell, AF3-NP was cleaved by cathepsin B to yield AF3-cleaved to turn the NIR-II fluorescence “On” for sensitive tumor imaging.
Topics & Concepts
FluorescenceMedicineIn vivoFluorescence-lifetime imaging microscopyCleavage (geology)BiophysicsNanotechnologyBiomedical engineeringMaterials scienceOpticsFracture (geology)Composite materialBiologyPhysicsBiotechnologyProtease and Inhibitor MechanismsAdvanced biosensing and bioanalysis techniquesAdvanced Proteomics Techniques and Applications