Obg-like ATPase 1 (OLA1) overexpression predicts poor prognosis and promotes tumor progression by regulating P21/CDK2 in hepatocellular carcinoma
Shanzhou� Huang, Chuanzhao Zhang, Chengjun Sun, Yuchen Hou, Yixi Zhang, Nga Lei Tam, Zekang Wang, Jia Yu, Bowen Huang, Hongkai Zhuang, Zixuan Zhou, Zuyi Ma, Zhonghai Sun, Xiaoshun He, Qi Zhou, Baohua Hou, Linwei Wu
Abstract
BACKGROUND: Obg-like ATPase 1 (OLA1) has been found to have a dual role in cancers. However, the relationship between OLA1 and hepatocellular carcinoma (HCC) remains unclear. RESULTS: High expression of OLA1 in HCC was detected in public datasets and clinical samples, and correlated with poor prognosis. Downregulation of OLA1 significantly inhibited the proliferation, migration, invasion and tumorigenicity of HCC cells. Mechanistically, GSEA showed that OLA1 might promote tumor progression by regulating the cell cycle and apoptosis. In addition, OLA1 knockdown resulted in G0/G1 phase arrest and high levels of apoptosis. OLA1 could bind with P21 and upregulate CDK2 expression to promote HCC progression. CONCLUSIONS: Overall, these findings uncover a role for OLA1 in regulating the proliferation and apoptosis of HCC cells. MATERIALS AND METHODS: The Cancer Genome Atlas and Gene Expression Omnibus datasets were analyzed to identify gene expression. Immunohistochemistry staining, western blot and real-time polymerase chain reaction were performed to evaluate OLA1 expression in samples. Cell count Kit-8, wound-healing, transwell and flow cytometry assays were used to analyze HCC cell progression. Subcutaneous xenotransplantation models were used to investigate the role of OLA1 in vivo. Coimmunoprecipitation was used to analyze protein interactions.