Pneumoperitoneum Modifies Serum and Tissue CCL2-CCL5 Expression in Mice
Alfonso Papparella, Carmine Noviello, Sara Ranucci, Orlando Paciello, Serenella Papparella, Davide De Biase, Grazia Cirillo, Giuseppina Rosaria Umano
Abstract
BACKGROUND AND OBJECTIVES: pneumoperitoneum in rats. METHODS: of 6 and 10 mm Hg, respectively. In the control group (C, n = 5), only anesthesia was carried out. All animals were sacrificed after 24 hours. The serum of the rats was collected for ELISA, and the levels of the cytokines RANTES and MCP-1 were investigated. An immunohistochemical analysis of RANTES and MCP-1 was performed on samples of the peritoneum, and the morphological evaluation was conducted with a blinded evaluation by two independent, experienced pathologists by using a grading system (0, 1+, 2+, 3+: no, faint, moderate, and strong reactivity, respectively). RESULTS: = .159). Normal control peritoneum showed little reactivity, whereas a moderate to strong cytoplasmic reaction to anti-CCL5/CCL2 antibodies was observed in mesothelial and inflammatory cells in the S1 and S2 groups. CONCLUSION: pneumoperitoneum evokes an inflammatory response by modifying plasma RANTES levels and peritoneal CCL5/CCL2 expression.