Lineage-Specific Growth Curves Document Large Differences in Response of Individual Groups of Marine Bacteria to the Top-Down and Bottom-Up Controls
Lívia Kolesár Fecskeová, Kasia Piwosz, Danijela Šantić, Stefanija Šestanović, Ana Vrdoljak Tomaš, Martina Hanusová, Mladen Šolić, Michal Koblížek
Abstract
hybridization (FISH). However, these laborious techniques are limited in their throughput and taxonomical resolution. Therefore, we combined a classical manipulation experiment with next-generation sequencing to resolve the growth dynamics of almost 300 bacterial phylogroups in the coastal Adriatic Sea. The analysis documented that most of the phylogroups responded positively to both grazer removal and phosphate addition. We observed significant differences in growth kinetics among closely related species, which could not be distinguished by the classical FISH technique.
Topics & Concepts
PhylotypeAnoxygenic photosynthesisBacterioplanktonBiologyPhototroph16S ribosomal RNAMicrocosmFlavobacteriaceaeEcologyNutrientZoologyBacteriaPhytoplanktonBacteroidetesGeneticsMicrobial Community Ecology and PhysiologyMarine and coastal ecosystemsIsotope Analysis in Ecology