Reply to Wang et al.: Clear evidence of binding of Ox to the oxygen-evolving complex of photosystem II is best observed in the omit map
Mohamed Ibrahim, Nigel W. Moriarty, Jan Kern, James M. Holton, Aaron S. Brewster, Asmit Bhowmick, Uwe Bergmann, Athina Zouni, Johannes Messinger, Vittal K. Yachandra, Junko Yano, Holger Dobbek, Nicholas K. Sauter, Paul D. Adams
Abstract
Wang et al. (1) performed their own analysis of our crystallographic data (2) and questioned our, and similar previous (3⇓–5), results that a newly inserted water (Ox) in the catalytic center of photosystem II (PSII) can be uniquely identified in the room temperature crystal structures taken at various time points during the S2-to-S3 transition of the water oxidation reaction in PSII. They suggest that the results can be explained by the movement of the existing ligand, O5, without incorporating a new water (Ox). The following four points describe why their claim is not valid. [↵][1]1To whom correspondence may be addressed. Email: jyano{at}lbl.gov. [1]: #xref-corresp-1-1