Label-free monitoring of 3D cortical neuronal growth in vitro using optical diffraction tomography
Ariel J. Lee, Dongjo Yoon, Seung Yun Han, Hervé Hugonnet, Weisun Park, Je‐Kyun Park, Yoonkey Nam, YongKeun Park
Abstract
primary neuronal cultures. A coupled confocal microscopy and immunofluorescence labeling are widely utilized for visualizing the 3D structures of neurons. However, this requires fixation of the neurons and is not suitable for monitoring an identical sample at multiple time points. Thus, we propose a label-free monitoring method for 3D neuronal growth based on refractive index tomograms obtained by optical diffraction tomography. The 3D morphology of the neurons was clearly visualized, and the developmental processes of neurite outgrowth in 3D spaces were analyzed for individual neurons.
Topics & Concepts
Optical coherence tomographyTomographyOptical imagingDiffuse optical imagingOptical tomographyIn vitroOpticsBiomedical engineeringMedicineBiologyOphthalmologyBiochemistryPhysicsDigital Holography and MicroscopyOptical Coherence Tomography ApplicationsAdvanced X-ray Imaging Techniques