A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice
Lila S. Nolan, Qingqing Gong, Heather N Hofmeister, Misty Good
Abstract
Recapitulating human NEC using animal models has been insightful in dissecting the signaling pathways, immune-mediated mechanisms, genetic signatures, and the intestinal architecture of NEC. This protocol describes an in vivo murine NEC model, using hypoxia and formula containing lipopolysaccharide and enteric bacteria derived from an infant with NEC. With this mouse model, we aim to further dissect NEC pathogenesis and develop new therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Mihi et al. (2021).
Topics & Concepts
Necrotizing enterocolitisImmune systemIn vivoComputational biologyProtocol (science)BiologyPathogenesisMedicineImmunologyBioinformaticsPathologyGeneticsInternal medicineAlternative medicineInfant Nutrition and HealthViral gastroenteritis research and epidemiologyClinical Nutrition and Gastroenterology