The Aspergillus fumigatus Secretome Alters the Proteome of Pseudomonas aeruginosa to Stimulate Bacterial Growth: Implications for Co-infection
Anatte Margalit, James C. Carolan, David Sheehan, Kevin Kavanagh
Abstract
The growth of Pseudomonas aeruginosa increased exponentially when exposed to the culture filtrates produced by Aspergillus fumigatus and by cocultures of A. fumigatus and P. aeruginosa in a nutrient-poor, nitrate-rich medium. Qualitative proteomic analysis of the A. fumigatus culture filtrates identified several secreted proteases and peptidases, including known human allergens. LFQ proteomics performed on P. aeruginosa exposed to the culture filtrates identified changes in several pathways and processes including an increase in outer-membrane proteins and stress response proteins.
Topics & Concepts
Aspergillus fumigatusPseudomonas aeruginosaMicrobiologyProteomeCystic fibrosisBiologyBacteriaPathogenAllergic bronchopulmonary aspergillosisImmunologyBiochemistryGeneticsImmunoglobulin EAntibodyBacterial biofilms and quorum sensingCystic Fibrosis Research AdvancesProbiotics and Fermented Foods