High-Definition, Video-Rate Triple-Channel NIR-II Imaging Using Shadowless Lamp Excitation and Illumination
Yuhuang Zhang, Shiyi Peng, Jian Guo, Jiayi Li, Zeyi Lu, Tianxiang Wu, Liying Chen, Wen Liu, Zhe Feng, Mingxi Zhang, Jun Qian
Abstract
Multichannel imaging in the second near-infrared (NIR-II) window offers vital and comprehensive information for complex surgical environments, yet a simple, high-quality, video-rate multichannel imaging method with low safety risk remains to be proposed. Centered at the superior NIR-IIx window of 1400–1500 nm, triple-channel imaging coordinated with 1000–1100 and 1700–1880 nm (NIR-IIc) achieves exceptional clarity and an impressive signal-to-crosstalk ratio as high as 22.10. To further simplify the light source and lower the safety risk, we develop a type of in vivo multichannel imaging-assisted surgical navigation mode at a video frame rate of 25 fps under shadowless lamp excitation and illumination instead of extra excitation light sources. This work provides a reference for real-time, high-imaging-performance multichannel imaging with minimal crosstalk and introduces a practical fluorescence surgical navigation paradigm.