Exploiting the unique properties of nanobodies: enhancing therapeutics, drug delivery, and targeted diagnostics
Lisa Blank, Giulia Pander, Eric Mühlberg, Walter Mier, Philipp Uhl
Abstract
• Nanobodies are small, stable molecules, with high binding specificity. • Proved potential for research and therapeutic applications. • Enable difficult applications where conventional antibodies fail. • Offer strong potential for diagnostic applications. Nanobodies (Nbs) are genetically engineered single domain antibodies derived from heavy chain-only antibodies (HcAbs) found in camelid species. These monomeric antibody fragments are smaller and have lower molecular weight compared with conventional antibodies, while retaining full antigen-binding specificity, endowing them with unique structural and functional properties. As in vitro diagnostics, they can aid pathogen detection and biomarker identification. In vivo , their rapid clearance and deep tissue penetration enable radio-imaging with short half-life radionuclides, reducing patient exposure. Therapeutically, Nbs are being explored for cancer, neurodegenerative, and infectious diseases. However, their short serum half-life is challenging, prompting strategies to extend circulation time without compromising their benefits. Despite these hurdles, the high specificity, low immunogenicity, and versatility of Nbs position them as promising tools across diverse applications.